Plasmid

Part:BBa_J119309:Design

Designed by: Brandon Grieshaber and Todd Eckdahl   Group: Eckdahl Lab   (2013-07-11)


pSB1A2-BR-HS-G-RNA-I


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2058
    Illegal XbaI site found at 2073
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of hotspot mutations was performed for the purpose of changing the plasmid copy number.


Source

Junction Golden Gate Assembly using BsaI, T4 DNA Ligase, inverted PCR of pSB1A2-BR and annealed oligonucleotides.

References